FastQCFastQC Report
Sat 24 Mar 2018
HJNFCBGX5_n01_1_cut16h-1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNFCBGX5_n01_1_cut16h-1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508706
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5920411.638156420407858No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG41070.807342551493397RNA PCR Primer, Index 27 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT6300.053.11602446
GTATGCC6400.052.8251142
TGCCGTC6550.052.66875545
AAGGGGG6400.052.31693669
TATCTCG6050.051.3193936
CTCGTAT6550.051.0886939
ATCTCGT6200.050.6342137
CGTATGC6700.050.45981241
CCGTCTT6650.050.3204447
TATGCCG6800.050.2250843
TAGGGGG1100.050.1789554
CGTCTTC6800.049.71775448
ATGCCGT7150.048.24944
TCTCGTA6600.048.0881738
TCGTATG7200.046.95565840
GTCTTCT7200.046.95565849
AGGGGGG2500.045.5374075
CTATCTC7150.043.42410335
CCTATCT7200.043.12254334
CTGCTTG8000.042.26424854
Produced by FastQC (version 0.11.2)