FastQCFastQC Report
Mon 8 Nov 2021
HJMY5DRXY_n01_dpr461.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMY5DRXY_n01_dpr461.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10850
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCCGT4243.907834101382489No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCGCGTATGCCGT3803.5023041474654377No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCC910.8387096774193548No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCCG730.6728110599078342No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCGCGTATGCCG680.6267281105990783No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCGCGTATGCC650.599078341013825No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCC480.44239631336405527No Hit
ATACACATCTCCGAGCCCACGAGACGAGGGGTTATCGCGTATGCCGTCTT310.2857142857142857No Hit
ATACACATCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCCGTCTT240.22119815668202764TruSeq Adapter, Index 10 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGC190.17511520737327188No Hit
TCTCCGAGCCCACGAGACGAGGGGTTATCTCGTATGCCGTCTTCTGCTTG160.14746543778801843TruSeq Adapter, Index 10 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGAGGGGTTATCGCGTATGCCGG160.14746543778801843No Hit
TCTCCGAGCCCACGAGACGAGGGGTTATCGCGTATGCCGTCTTCTGCTTG120.11059907834101382Illumina PCR Primer Index 10 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)