FastQCFastQC Report
Wed 15 Dec 2021
HJMVWDRXY_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMVWDRXY_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120097417
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTCAC717056183.382185055508702No Hit
TGGTGTTT697846373.2915769077605552No Hit
CAGCCCAC693416283.2706812170037187No Hit
GGCGTTAT688452313.2472673400743077No Hit
ATTGGCGT677387913.195079172156739Illumina PCR Primer Index 6 (100% over 8bp)
TCCTCCAA677232263.1943450077794235No Hit
CATGCGAT669371543.157267843603057No Hit
CTACATCC653983873.0846878297007994No Hit
ACCCGACG640620313.0216550657681407No Hit
TAGACACC634837172.99437735695331No Hit
CGCCATCG633824182.989599321793806No Hit
ATTCCGAT633186732.9865926203333513No Hit
CCACAGGG624889202.947455126303755No Hit
TAGTTGTC622194192.9347433991048533No Hit
TGGATTGC621087272.9295223182661894No Hit
ATTTGCTA620207322.925371801441141No Hit
CCCTAACA618534412.917481079125337No Hit
TTTGGGTA616686322.9087640740236798No Hit
GCAACAAA608184312.8686620960116005No Hit
GATCAGCA606931872.862754631618892No Hit
GAAGGCTG605580482.856380443389786No Hit
GGCAATGG604484562.8512112469594126No Hit
GGGGGGGG600770662.8336936556911057No Hit
AGGAGATG600299272.8314702201252673No Hit
GATGTGGT599563982.8280020304368874No Hit
TGATATTC592816082.7961737760090863No Hit
CCGGGTAT591226692.788676997855142No Hit
ACAACAGG589801852.781956363281584No Hit
ACATTACT558817222.63580916385787No Hit
GTGATCGA542403742.5583906458766275No Hit
TAACAAGG526485192.483306596094966No Hit
GGTTCCTC521304372.458869888807567ABI Solid3 Adapter A (100% over 8bp)
ATCATGCA515260722.4303634157014775No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)