FastQCFastQC Report
Fri 22 Oct 2021
HJML5DRXY_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJML5DRXY_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199052549
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGCGC971118114.416075052147378No Hit
AATCTCTC944906714.296880992815238No Hit
TCAGAAAT906310664.12136881591182No Hit
CACCGCTC890123404.047758660450274No Hit
GAGACTAT881065114.006566875360285No Hit
CGGAGGGA873761393.9733538445788184No Hit
TATGGTGT864698333.9321403683291427No Hit
TCGATACT862672853.9229296743831474No Hit
ATAGGCCA815558203.708679905675142No Hit
GTCTCTCG812994953.697023749476575No Hit
AGATCTGG801281053.643755809129598No Hit
ATGACCGC786476723.5764344074344354No Hit
CGCTTAGC733331133.334759464176861No Hit
GTTGAGAA708382053.221305695137347No Hit
TCTCGTTT703512383.1991612948036012No Hit
CGAACCTG683679603.108973454549312No Hit
TCTCAGTG672620773.0586843879918577No Hit
GAGGATCT672466153.057981267004275No Hit
CAAGTAAA665659073.027026663381476No Hit
GCGCAGAA645650212.9360381146580776No Hit
GGCTAGCG642672202.922495873471735No Hit
CTTATGAG640624222.913182862734764No Hit
AGACCATA629072542.860652603713655No Hit
ATCTTACC622810312.832175658026988No Hit
CACTCGGA544666092.476821621419107No Hit
GCTGAATT538422932.4484313948970575No Hit
GGGGGGGG518306032.35695154368046No Hit
TGAAGTAC517110932.3515169304851384No Hit
ATGCTCCG508738042.313441942218908No Hit
CTTAGGAG82896320.37696379760318316No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)