FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71996575
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC1401640.19468148311221747No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1217920.16916360257415022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCCC165600.056.140891
TGCCCCC58800.044.1947561
TCGCCCC49200.039.2037351
ACCCCCC281550.038.700411
TGGCCCC55800.035.6351621
GCCCCCC1402800.016.3472791
TGGGGGG100250.016.1617151
TGCGCCC28400.015.5590131
TGGGGGT60950.014.4996221
GGGGGCA32550.013.2289542
GGCCCCC248800.011.9743132
TGGGCGC15500.011.7652611
GGGCCCC103900.011.678591
TGGGGGC57250.011.148741
GGGGGAT68600.010.7866911
GGGGCCC71600.010.3347341
TCCGCCC69500.010.2433281
GGGGGGT59650.010.2119862
GGGGGTG76350.010.0416832
CGGGCAT45100.09.8754531
Produced by FastQC (version 0.11.5)