FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_9_NxW_C3-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_9_NxW_C3-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48928247
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT57200.012.1172261
CGGGAAT128750.09.8694611
CCGGGAT117150.09.6813691
CGGGCGC19550.09.66744715
CGGGGAT85750.09.3483591
CGCCCAT80650.08.8978181
CGGCAAT122300.08.8729761
CCGGCAT80650.08.7676061
CGCGGAT70450.08.6954311
GCGGGCG32650.08.5757466
GGGGGAT73900.08.5736981
CGCGTAT40700.08.42881
CTCGGAT221050.08.361371
GTCGGAT177400.08.1297591
CGCGGGC34800.08.04591765
GGGAGAT259650.08.0081491
CGGGGAG72650.07.9502791
GCCGGAT110400.07.92692851
CCCGTAT51250.07.92315531
GCCAGAT148150.07.8209661
Produced by FastQC (version 0.11.5)