FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_8_NxW_C3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_8_NxW_C3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30078565
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT38000.013.5416541
CGGGGAT50100.011.0397011
GCCGGAT68050.010.44253351
CGGGCGC14750.09.72872915
CGGGAAT83750.09.1537121
CGCGTAT23800.09.1191151
CCGGCAT49950.09.1105761
CGCCCAT51800.09.0555111
GCCAGAT89600.08.9076831
GCCGAAT43950.08.5224181
GCGGGAT70250.08.4711141
ATCGGGC28650.08.4293316
CCGGGGT40800.08.3224181
GGGAGAT164900.08.2153921
CGCGAAT44000.08.1945011
CGGGGAG48750.08.1859331
GGAAAAT256000.08.1360761
GGGAAAT161700.07.8151111
CCCGCAT32450.07.7670441
GGAAGAT346950.07.7386721
Produced by FastQC (version 0.11.5)