FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_7_NxW_C3-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_7_NxW_C3-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19907434
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT26050.011.959681
GCCGGAT42150.010.4643011
GCCGAAT28400.010.2305041
CGGGGAT34700.010.1889441
CCGGGAT51150.09.2390091
CGGCAAT50450.09.1590411
CGGGAAT55150.09.1401691
CGGGGAG31900.08.99831
GCGGGAT41250.08.7407871
GCGCGAT10600.08.5862741
CGCCCTT20300.08.4496221
CCGGCAT34100.08.4177641
CCCGCAT20850.08.3946231
CGCGGAT32350.08.3320861
CGCGGCT37500.08.3079911
CGGGTAT32900.08.2991941
GGAAAAT164650.08.185331
GGGGGAT30000.08.1679691
GCGAAAT56500.08.0543811
GCGAGAT53050.08.0502941
Produced by FastQC (version 0.11.5)