FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_6_NxW_C2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_6_NxW_C2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34942629
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT62000.013.1555131
CGGGCAT42350.012.6468271
GCCGGAT78850.010.7437821
CGGGAAT89350.010.1472591
CCGGGAT89350.09.9905441
GGGGGAT56400.09.9308041
GCGGGAT66300.09.8735111
CGGGGAG47900.09.5736891
CGCGGAT52500.09.4016351
GCCGAAT46550.09.3249291
CCGGCAT58100.09.0377161
CGGGGCT38650.08.966631
GGGGAAT85950.08.9195251
CGGCAAT80450.08.8331081
GCCAGAT106200.08.6691151
GTCGGAT132250.08.6026291
GCCGGGT73900.08.5265161
CCGGGAC52800.08.4200241
GCCGGGG32000.08.3139451
GTCGAAT127700.08.2512381
Produced by FastQC (version 0.11.5)