FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_5_NxW_C2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_5_NxW_C2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15345822
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT25700.010.7606941
CGGGCAT16100.010.6540931
GCCGGAT34750.010.3759691
CGGGGAG19950.09.8263311
GGGGGAT22850.09.804831
GGCGCCC5855.914808E-89.57256567
CGCGGAT21600.09.5619081
CGCCCAT24200.09.2578661
CGGGGCT16100.09.1320791
CGGGCAC16900.09.1140671
CGGGAAT41950.09.09579851
GCCGAAT19950.08.9489791
GCCAGAT46900.08.8821971
GGGCCCT19050.08.81881968
GCGGGAT31450.08.7933171
GTCGGAT61200.08.7515761
CGGGTAT21900.08.6316921
CGCGTAT9454.5474735E-118.5200531
GCGCGCG4956.4381114E-58.48477464
CGCCCCT7858.269126E-98.4728641
Produced by FastQC (version 0.11.5)