FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_4_NxW_C2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_4_NxW_C2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24925129
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT30600.013.384531
CGGGGAT41150.012.2498661
CGGGGGT13250.011.6245321
GCCGAAT33600.010.8350941
GGGGGAT41300.010.5101831
CCGGGAT63700.010.4412581
CGGGAAT68600.09.848541
GCGGGAT49200.09.605221
CGGCAAT63150.09.5344091
GGCGCGC10350.09.46852438
GCGGGCT20650.09.323551
GCCGGAT55700.09.1756391
GCGGAAT48900.09.1630451
CGCCCTT23900.08.788041
GCGGGTT56300.08.7669671
CCGGCAT42550.08.72057151
CGCCCAT39850.08.6965211
GCCGGGT58100.08.676111
GTCGGAT93050.08.4645681
CGCGAAT34050.08.4301521
Produced by FastQC (version 0.11.5)