FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_47_NxW_C16-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_47_NxW_C16-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14715734
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT15200.013.5875441
GGCGCGC7600.013.3551638
GGGGGAT30950.013.1198821
GCGCGCA9500.011.05254639
CGCGCAC9500.010.68412840
CCGGCAT22850.010.2641071
CGCGGAT21200.010.072251
CGCCCAT28900.010.0533961
GGGGATA40450.09.95179752
CCCCACT16200.09.9397481
GTCGGAT66900.09.7847111
GCCGGAT30850.09.7583371
CGCGTAT12600.09.7236671
CGGGAAT46500.09.6358391
CGGGCCC4451.8364153E-59.4396041
ACCGCTA29200.09.2293849
GGCGCCC10800.09.07400467
CGGGGAT25950.08.9030571
CCAGTCG27350.08.82991363
CGGGAAC61100.08.7656231
Produced by FastQC (version 0.11.5)