FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_46_NxW_C16-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_46_NxW_C16-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36183622
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC17300.014.16173138
GGGGGAT71750.011.3190121
CGCGTAT30150.010.797871
GCGCGCA22800.010.74552339
CGGGCAT34900.010.2309791
CGCCCAT68350.010.1919431
GCGGGAT59450.010.1278911
CGCCAAT78350.09.7400221
CGCGGAT53750.09.7039881
GCCGAAT46100.09.6437341
CGCGCAC24950.09.53899940
CCCCGCT21200.09.4119891
CCCGTTT73900.09.3791441
GTCGGAT164850.09.1310751
CCGGGAT97450.09.124191
GTCGCAT45150.09.0713211
CGGACAG53750.09.05108717
CGGCAAT78750.08.8015071
GCCGGAT78150.08.7794941
GTCGATT117500.08.7589471
Produced by FastQC (version 0.11.5)