FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_45_NxW_C15-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_45_NxW_C15-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26119919
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC12200.015.20477838
GGGGGAT51050.014.263021
CGGGCAT26550.012.5257271
GCGCGCA15950.011.84942139
CGCGCAC17300.011.12706640
TAAACGT93250.010.7729683
CGCGTAT19650.010.6888891
GGGGATA73100.09.8645652
CCGGCAT37650.09.8556381
TGCACTA95300.09.84251222
ATCGCAC39150.09.6551768
GCCCGAT17350.09.4829231
CCCACAT51150.09.4444641
GCACTAC102600.09.27866723
TTAAACG110050.09.2243552
CGTTTTA102950.09.2132147
GCCGGAT57700.09.1610441
GCGGGAT39950.09.112981
CCCGCCT32750.09.0855551
CCCGTTT54350.09.0172141
Produced by FastQC (version 0.11.5)