FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_44_NxW_C15-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_44_NxW_C15-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37976444
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT40050.012.062011
GGCGCGC16300.011.16554538
GGGGGAT64450.010.1569151
CGCGTAT30500.09.9853321
CGCCCAT70450.09.440951
CGAACGC47550.09.4223973
CGCGGAT60050.09.3271731
CGCCAAT80250.09.20408251
GCCGAAT48550.08.9407831
GCGGGAT63200.08.917681
ATCGCAC58250.08.7725128
CCGGCAT59600.08.7515131
CCCGTTT80950.08.562321
ACGCACC46700.08.3940186
GGGTCGC30450.08.3907347
TAAACGT119950.08.3457963
GGGAGAT226300.08.337721
GTCGGAT170850.08.2981861
GCGCGCA23200.08.2973439
GAAAAAC295800.08.295891
Produced by FastQC (version 0.11.5)