FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_43_NxW_C15-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_43_NxW_C15-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61426032
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAT105850.012.0706721
GGCGCGC26950.011.94797138
CGGGCAT59950.011.911611
CGGGCCC13850.010.1097331
CGCCCAT110950.010.0645151
CGCCAAT130650.09.8597951
GCGCGCA34850.09.84211439
CCGGCAT95000.09.8013861
CGCGGAT93600.09.7609961
CGCGCAC37800.09.16660540
GCGGGAT98100.08.9207321
CGAACGC73300.08.8818073
GTCGGAT279250.08.7998121
CGCGTAT52050.08.7428311
GGGGATA150100.08.6519122
GCCGGAT129400.08.6295051
CGGGGGT22800.08.5977081
GCGCGAT30200.08.5773731
CGGGGAT103100.08.5560111
ATCGCAC95050.08.5428298
Produced by FastQC (version 0.11.5)