FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_42_NxW_C14-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_42_NxW_C14-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41611750
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC19850.011.98983538
CGGGCAT45150.010.6217851
CGCGCAC24900.010.26096740
GCCGAAT57400.010.1235061
CGGGAAT125500.09.8740251
GGGGGAT76850.09.4289041
CGCCAAT91200.09.4038561
CCCGTTT87600.09.3907111
GCGCGCA27100.09.16967339
CGCCCAT79850.09.0308161
GTCGCAT47300.09.028871
CGCGTAT35400.08.9985571
GCCGGAT87000.08.9726411
GTCGGAT204850.08.6466771
GCCAGAT115350.08.4364921
CGCGGAT65700.08.418341
CGGCAAT90900.08.3951271
CCGGCAT63850.08.3333071
CGGGGAT69050.08.3140931
ATCGCAC61500.08.3089218
Produced by FastQC (version 0.11.5)