FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_41_NxW_C14-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_41_NxW_C14-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20161120
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC8950.016.81551638
GGGGGAT34600.013.8606831
CGCGCAC11700.013.46143840
GCGCGCA12250.012.57133439
CGCCCAT37650.012.3659321
CCCGTTT45900.011.89741
CGGGCAT22400.010.47048951
CGCGTAT16350.010.2769371
CGGGGAT32750.010.1543581
GGGGATA46050.010.109882
GTCGGAT90800.09.9465861
CCCGCCT26950.09.7418781
CGCCAAT43550.09.6456891
TAAGCGG35250.09.63113448
CGCGGAT32950.09.4552891
GCGCACC16850.09.34711141
CGGACAG33400.09.32627817
CCGGCAT32300.09.2120561
GTCGCAT24100.09.0056451
CCCCGCT13650.08.9758491
Produced by FastQC (version 0.11.5)