FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_40_NxW_C14-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_40_NxW_C14-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38471000
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT42700.012.4608491
GGGGGAT69450.012.3992321
GGCGCGC18450.011.38202838
CGCCCAT72050.010.8829341
CCCGTTT81650.010.6751771
CGCGTAT31200.010.3220311
CGGGAAT121600.09.2406681
GCGGGCG22300.09.10306914
GTCGGAT173550.09.056361
TCGGGCG26300.09.0493967
CCCCGCT29450.09.03358651
ATCGGGC28800.08.9930236
CGCGCAC23550.08.917129540
CGCCAAT80900.08.9135481
CCGGGGT106700.08.89071751
GCCGGAT79700.08.8281491
CGCGGAT58450.08.7438011
CGGCAAT88400.08.7116891
GCGCGCA26150.08.69974339
GGCGGGC24700.08.6436613
Produced by FastQC (version 0.11.5)