FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_3_NxW_C2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_3_NxW_C2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23014748
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT31500.012.224281
CGGGGAT37800.012.0390641
GCCGAAT36850.010.9244991
GCGGAAT48350.010.2085451
GCCGGAT49550.010.0319621
GGGGGAT39100.09.8482061
CCGGGAT58150.09.7522871
CGCCCTT23300.09.4651141
CGGGAAT59850.09.4167911
CGCCAAT47450.09.3693341
CGCGTAT17150.09.1852211
CCGGCAT37350.09.0912241
GTCGAAT79800.08.9050081
GCGGGTT50000.08.8914981
CCCCGAT18650.08.8218611
GCGGGAT56800.08.6898441
GGCGAAT69800.08.5257911
CCCGACT43350.08.3981851
GGGGAAT64400.08.3709751
CCCGTAT27750.08.1995791
Produced by FastQC (version 0.11.5)