FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_39_NxW_C13-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_39_NxW_C13-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23026921
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT24600.011.66841
CGCCCAT38900.010.0786171
CGCGCAC11150.010.044764540
ACTCTAG63950.09.6879595
GTCGGAT89250.09.4523851
GGCGCGC13000.09.15377438
CGCCCTT25200.09.0291191
CTAGTTG55400.08.9078928
CGGGGAT39750.08.89440251
GCCGGAT53150.08.8912541
CCCGCCT30000.08.8679841
CTGTAGG45450.08.7788149
GCGGAAT46750.08.6109041
CGGGAAT59500.08.5895111
TACTCTA58550.08.5488694
GTAGGAC41450.08.52828151
CGGGGGT9901.2732926E-118.4861091
CTCTAGT64150.08.4566956
CCTACTA44000.08.35220734
CCGGGAT59600.08.3401641
Produced by FastQC (version 0.11.5)