FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_38_NxW_C13-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_38_NxW_C13-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47130830
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT54500.011.4331081
CGCCAAT93450.09.5147031
CGCGGAT64350.09.3566561
CGGGGAT74300.09.2343861
GCCGAAT58450.09.1033211
CGCCCAT78100.09.0988361
CCGGGAT119400.09.0299881
CGGCAAT101450.08.7644061
GTCGGAT192350.08.62634851
GCGGAAT91300.08.5885151
CGCGTAT33400.08.4894471
GCGGGCG24950.08.41676166
GCGGGAT85200.08.34061
CGGGAAT124350.08.3327191
GCCAGAT139200.08.3239531
CGGGCCC12400.08.1868551
GGGGGAT67800.08.1577091
GCGCGCG18900.08.14807864
GTCGAAT153250.08.0404991
CTCGGAT196600.07.941311
Produced by FastQC (version 0.11.5)