FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_37_NxW_C13-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_37_NxW_C13-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68158892
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT77400.010.809391
GCGCGCG24150.010.14483964
GGCGCGC31100.010.0159938
GGGGGAT106800.09.7020771
CGCGCAC34100.09.44273340
CGGGGGT30400.09.4424461
CGCCCCT38950.09.2570791
CGCCCAT108350.09.24021
GCGGGAT122600.09.2226621
GCCGGAT153700.09.2013541
CCGGCAT106600.09.162021
GCCGAAT87200.09.1128371
CGCGTAT47400.09.0100191
CGGGGAT107350.08.9675721
GTCGAAT260650.08.7565721
CGCGGAT90550.08.6983791
CGGGAAT176600.08.6623351
CGCCCTT68850.08.6434921
CCGGGAT175100.08.5566131
CCCGTTT141750.08.5200131
Produced by FastQC (version 0.11.5)