FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_36_NxW_C12-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_36_NxW_C12-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89293596
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT93550.012.7973751
GGCGCGC41250.011.11506638
GGGGGAT139300.010.378571
CGCCCAT146400.09.7556831
GCCGAAT109650.09.5455541
CGGGGAT151550.09.493461
CGCGGAT140800.09.2486611
GCGGGAT142500.09.064631
GCGCGCA53150.09.02156539
CGGGAAT254150.09.0079561
GTCGGAT379650.08.9715611
CGCGCAC52900.08.93187740
CCGGCAT146600.08.5006981
CCCGTAT75650.08.4679981
GCCGGAT201550.08.4062271
CGCCCTT85000.08.4013641
CGCGTAT68050.08.3334631
CCGGGAT239950.08.1405181
GCCAGAT255200.08.0930061
CGCCAAT189700.08.0271341
Produced by FastQC (version 0.11.5)