FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_35_NxW_C12-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_35_NxW_C12-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31969697
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT32900.012.7680711
GGCGCGC14200.011.58441438
CGGGGAT55950.010.1357261
CGCCAAT69250.09.9583261
CGCGTAT26550.09.8886431
GGGGGAT58550.09.6258461
CGGGAAT97300.09.5339521
CCGGCAT51300.09.4850011
TAAACGT105750.09.3009183
CGAACGC38750.09.2135783
ACGCACC43550.09.0011326
GCCGAAT42450.08.9060691
CGCGGAT48000.08.89730551
GCGGGAT49650.08.8836451
CGCCCAT55650.08.8693921
CGGGCCC6755.6197678E-88.8162741
GCCGGAT70000.08.8014561
ATCGCAC52150.08.7919038
TGCACTA103900.08.72466522
CGCGCAC20100.08.532269540
Produced by FastQC (version 0.11.5)