FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_34_NxW_C12-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_34_NxW_C12-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26676553
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT31500.012.55765151
CCGGCAT44400.010.32831
CGCGGAT43250.09.7935421
GGGGGAT43000.09.6876641
CCCACAT65800.09.0972631
CGGGCGC15450.08.83486915
CCGGGGT57300.08.6140031
CGGGAAT76050.08.6076161
CGCCAAT54550.08.5348811
CGGGGAT45600.08.5211591
GCCAGAT79300.08.5197081
GTCGGAT108500.08.420761
GCCGAAT36300.08.3898311
CGCCCAT47800.08.275441
GCGGGAT54750.08.1200771
CGGCAAT66650.08.0883721
CTCGGAT110700.08.0320551
CCCGTAT29250.07.8987551
GTCGAAT103800.07.8577661
GCCGGAT59900.07.77258351