FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_33_NxW_C11-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_33_NxW_C11-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19267008
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC8350.012.5747538
CGGGCAT21750.010.62231351
CCGGCAT30800.010.4561441
CGCCCAT36500.010.4536531
CGGGCCC4753.4290497E-710.3173671
GGGGGAT33300.09.881391
CGCGCAC11400.09.82448340
GCGCGCA11150.09.73086439
CCCGCCT22500.09.645921
CGCCCTT20800.09.4245181
CGGGGAG25700.09.3983241
GTCGGAT82300.09.3149121
CGCGTAT16550.09.3065591
CCCGCAT19650.09.2635021
CGGGGAT30400.09.2119351
TCGGGGG21550.08.9338492
CGCCAAT40100.08.7295141
GCCGGAT40600.08.7082281
GCGGGAT32000.08.5325551
CGGGCAC19700.08.52922251
Produced by FastQC (version 0.11.5)