FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_32_NxW_C11-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_32_NxW_C11-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44445626
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT47600.011.7670351
GGCGCGC19250.011.27261338
CGGGGAT73300.010.4590971
CGCGTAT32700.010.0631541
GGGGGAT73350.09.7360781
CGCGGAT65450.09.6810611
GCGGGAT71450.09.3580461
CGCCAAT94150.09.2583391
CCGGCAT72550.09.1679071
CGCCCAT75450.09.0475171
GTCGGAT180300.08.9507461
GCCGGAT97650.08.8547991
CCCGCCT56200.08.7828771
CGCGCAC26350.08.76651540
CGGGAAT120250.08.55886651
GCGCGCA26800.08.22752939
CCGGGAT124950.08.2089081
GCGGAAT88800.08.1209771
GCCAGAT134800.07.9986161
CGGGGAG54300.07.9942161
Produced by FastQC (version 0.11.5)