FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_31_NxW_C11-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_31_NxW_C11-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29050837
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT29200.012.1081731
GGCGCGC14750.011.15245538
ATCGCAC43500.010.7011398
CGCCCAT50300.010.1607321
GGGGGAT47550.09.7177021
CGGGAAT86050.09.4379411
CCCGTAT24150.09.2769011
ACGCACC38200.09.2539176
GCCGGAT61900.09.1614561
GCGCGAT15150.09.0113961
CCGGGAT81850.08.9813321
GCGGGAT51150.08.8968821
GTCGGAT125400.08.8491551
CGCGCAC19450.08.81741140
TCGCACA55800.08.7813539
GAAAAAC229900.08.6943531
CCCGTTT64150.08.6764211
CGAACGC38000.08.5663123
CGGGGAG40050.08.5657161
GCGCGCA20450.08.5573939
Produced by FastQC (version 0.11.5)