FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_30_NxW_C10-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_30_NxW_C10-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55364796
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC26050.012.09202438
CGGGCAT55700.011.0612071
GGGGGAT92950.010.5075111
CGCCAAT117650.010.4140861
CGGGGAT93850.010.1456441
GCGCGCA32800.09.60357439
CGCGGAT84250.09.556591
CGCGCAC32350.09.52078240
CGCGTAT40550.09.4098061
CCGGCAT85050.09.343221
GCGGGAT89000.09.0465471
CGGGGAG72550.08.7334571
CCCGTTT120750.08.7261841
CGCCCAT97950.08.6845411
GGGTCGC46050.08.66441847
GTCGGAT243250.08.6490151
CGGGAAT154350.08.57294751
GCCAGAT156800.08.57294751
ATCGCAC78900.08.4283628
ACCGCTA105900.08.29548949
Produced by FastQC (version 0.11.5)