FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_2_NxW_C1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_2_NxW_C1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29793783
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC15950.011.84943215
CGGGGAT46000.010.72991
CGGGCAT38200.010.2633481
CCGGCAT47500.09.7278021
GCCGAAT44000.09.6264711
GCCGGAT69150.09.3145111
CGGCAAT75050.09.2818981
CGCGTAT22650.09.2729411
GGGGGAT42000.09.2514141
CGGGAAT81250.09.0475361
CCGGGAT70600.08.8752941
CGCGGAT43100.08.6092041
GGAAAAT249550.08.4585161
ATCGGGC30650.08.4502886
GCGGGAT73700.08.2169941
CGCCCAT53350.08.2018161
GCGGGCG22100.08.07685714
GTCGGAT114000.08.0757951
GCGAAAT87050.07.8817331
CGCCCTT29900.07.8440081
Produced by FastQC (version 0.11.5)