FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_29_NxW_C10-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_29_NxW_C10-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28473941
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT29600.011.7076271
CGGGGAT48700.010.9254671
TGCGCTA28800.010.08673967
CGGGGCT24050.010.0429061
GGCGCGC11950.09.66520738
CGGGGAG38000.09.4880951
CGGGAAT78850.09.4115141
CGCGGAT45050.09.4019071
GGGGGAT49000.09.3583781
GCGGGAT50000.09.24121951
GCCGGAT62950.09.2307651
CATGCGC31600.08.97145865
CGGGGGT10700.08.8329421
GTCGGAT115450.08.6109241
CCGGCAT47750.08.503741
GCCCGAT21100.08.4608331
ACATGCG35650.08.44314164
CGCCCAT48300.08.3344341
GGGGAAT62300.08.3157041
GCCAGAT83400.08.310451
Produced by FastQC (version 0.11.5)