FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_28_NxW_C10-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_28_NxW_C10-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29260476
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT32350.011.1455741
CGGGGAT50200.010.7388171
ATCGCAC41050.09.6345468
CGGGGAG41450.09.5431851
GGGGGAT48900.09.3778211
CGCGTAT21700.09.3563781
CCGGCAT43950.09.3189391
GGCGCGC12950.09.18911538
GTCGGAT119900.08.9923041
CGCCAAT63300.08.8482161
GCCGGAT62600.08.8353191
CGCGCAC15800.08.6391740
CGGGAAT84450.08.6219031
GCCGAAT38050.08.555941
TCGCACA48100.08.51347359
CGGCAAT64800.08.373291
TAAACGT90050.08.3182153
ATCGGGC19150.08.2245326
TGCGCTA30050.08.15301267
GCCAGAT84600.08.1100811
Produced by FastQC (version 0.11.5)