FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_27_NxW_C9-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_27_NxW_C9-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27329500
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT56300.010.3216181
CGGGCAT33100.09.9414091
CGGGGAT45250.09.8250151
CGCGGAT36150.09.7805011
CGGGCGC9400.09.68075715
CCGGGAT64900.09.6011511
GCCGGAT66650.09.4015821
GCCGAAT41650.09.1613521
CGGGAAT73750.08.7812791
GCCAGAT91050.08.7275821
GGGGAAT71750.08.4405781
GTCGGAT89000.08.3386141
CCGGCAT49550.08.3365511
CGGGGAG39200.08.3051011
CCCGCAT31200.08.3028121
CGCGCAT17850.08.2368131
CCCGACT43000.08.2224451
CGGGGCC9001.3187673E-98.1681721
CGCCAAT57600.08.0830861
CGCCCAT49500.07.9913711
Produced by FastQC (version 0.11.5)