FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_26_NxW_C9-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_26_NxW_C9-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11322493
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT11500.012.4809261
CGCGTAT7001.0004442E-1010.0021351
GTCGGAT44100.09.9227531
CGGGAAT26450.09.6617981
GGGGGAT14500.09.6572341
CGCGGAT13450.09.6303091
CGGGGAT19000.09.5809931
GCGGGAT18300.09.5648821
CCCGCAT10400.09.4250891
GCCGGAT25650.09.4172151
CCCCGAT9750.09.3353261
GTAGGAC21750.09.33322451
ACTCTAG31550.09.2085975
GCGGGTT22500.09.1797381
CGCCCAT17550.08.97627451
CTAGTTG28600.08.933548
GTCGAAT39400.08.8851451
TACTCTA29950.08.765554
GGGAAAT58900.08.737011
GCCCGCT6958.997449E-88.5629791
Produced by FastQC (version 0.11.5)