FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_25_NxW_C9-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_25_NxW_C9-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26389050
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT31400.011.594041
CGCGGAT31650.010.8388571
CGGCAAT60500.010.0097181
CGGGGAT44950.09.5008221
CGCCCCT15250.09.1816611
CCGGGAT61500.08.9362561
GCCCGAT24400.08.7512691
CGCGTAT19850.08.6410531
GTCGGAT82100.08.5700621
GCGCGCG11450.08.55889764
CGCCCAT45300.08.4228551
CCGGCAT47500.08.4012191
CGGGAAT66250.08.2427061
GCGCGAT13250.08.1898681
CCCGCCT34600.08.0936591
CTCGGAT114200.08.0922421
TAGCGCG15300.08.00648562
GCCGGAT62150.07.99794341
CGCACAT32100.07.9606571
CCCGCAT29100.07.93929671
Produced by FastQC (version 0.11.5)