FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_24_NxW_C8-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_24_NxW_C8-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22251396
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT28300.012.7402071
CGGGGAT38000.010.961981
CGGGAAT62450.010.7059841
GCCGAAT32100.010.1415321
GGGGGAT36350.09.9187851
GCGGGAT51150.09.5125031
CCGGGAT57800.09.3264961
GGGGAAT56250.09.2101111
CGCCCAT38200.09.0718841
CCGGCAT35250.08.8380511
CGGCAAT58550.08.7885271
GTCGGAT89850.08.5320171
CCGCAAT41850.08.5315971
GCGGAAT44300.08.4548461
GTCGAAT75750.08.2717251
CGGAAAT86900.08.2174311
GCCGGAT49750.08.1618861
CGCCTAT17850.08.0402821
CGGGGGT11801.8189894E-128.0095371
CCCACAT52800.07.8893041
Produced by FastQC (version 0.11.5)