FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_23_NxW_C8-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_23_NxW_C8-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15622683
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT16650.011.773811
CGGGGAT25300.010.1005631
GCCGGAT36550.09.6733581
GCCGAAT22500.09.6461151
CGCCAAT33600.09.4808081
CCGGCAT28300.09.1535291
CGGGCGC5058.319288E-69.00981715
GTCGGAT56300.08.8292381
GCGGGGT9152.1827873E-118.7993381
CCGGGAT39400.08.7959391
GGGGGAT24800.08.7515151
CGCGGCT23050.08.6565971
GCGGGTT31600.08.6407371
CGCCCAT22500.08.5570381
CGGGAAT39300.08.5510991
CCGGGGT19400.08.4808511
CGGACAT27250.08.4785331
CGGGGGT7553.8369762E-88.3458171
GGGGAAT38650.08.332621
GCCAGAT53500.08.2444181
Produced by FastQC (version 0.11.5)