FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_22_NxW_C8-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_22_NxW_C8-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87365782
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT156550.010.822561
CGGGCAT101750.010.5962951
CGGGAAT233950.010.0251181
GCCGAAT118850.09.6902381
GCGGGAT150800.09.2388831
CCGGGAT217650.08.9906451
CGGGGGT42350.08.92705251
GCCGGAT203550.08.7707511
CCGGCAT156200.08.7401991
GGGGGAT135700.08.6933631
CGCGGAT127950.08.6453891
GCGGGCT62500.08.5693751
CGCGGGT66400.08.4350891
GTCGGAT308600.08.3827451
GCGGGTT180400.08.3439111
CGCCCAT127150.08.314351
GCCAGAT288400.08.2901651
CGGGGAG107500.08.1082771
CCGGGGT127450.08.1025171
GCGGAAT187550.08.1004731
Produced by FastQC (version 0.11.5)