FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_21_NxW_C7-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_21_NxW_C7-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40944848
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT44150.011.7348341
CGGGGAT70150.010.4794271
GCGGGAT70500.09.5832791
CGGGAAT106800.09.5710051
CGGGGGT17300.09.3080271
CGCCCAT64400.09.2951451
GCCGGAT93350.09.1875071
CCGGCAT71750.09.1235881
GCCGAAT55700.08.9244011
GTCGGAT148600.08.8340211
CGCGCAT24500.08.7158481
CCGGGAT102850.08.5771341
GGGGAAT95250.08.4897111
GCGGAAT87600.08.3919161
CGGGGCT35500.08.2831751
CGGCAAT104100.08.2723771
GGGGGAT59800.08.2539891
GCCAGAT128150.08.1130431
GCGGACT27900.08.0301131
CGCGTAT32350.08.0076191
Produced by FastQC (version 0.11.5)