FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_20_NxW_C7-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_20_NxW_C7-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20022026
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT35750.012.0436521
CGGGCAT22950.011.8970981
GCCGAAT27500.011.7107371
CGCGGAT29750.010.8250511
CGGGAAT54550.09.6897221
CGGGCCT15750.09.5568991
GCCGGAT47250.09.408731
CGGGGGT7901.0186341E-109.3051061
CGGGTAT29600.09.2242691
CGGCAAT53350.09.1859211
CCGGCAT34300.09.082911
GTCGGAT76850.09.0188351
CCGGGGT31900.08.998131
GCGCGAT10250.08.8792971
CGGGGAG26550.08.7017891
GCCAGAT59500.08.4717791
GGAAAAT163900.08.3934921
CGCCCCT9656.91216E-118.3431421
GCGGGCT13550.08.2668451
GCGGGAT34300.08.2664681