FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_1_NxW_C1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_1_NxW_C1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22564857
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCAT37300.010.8867251
CGGGCAT29050.010.3633561
CGGGGAT35400.09.8888291
GTGTGCG18000.09.3332758
CGCCCAT41550.09.26765251
CGCGTAT17800.09.2432761
CGCGGAT35150.08.8636531
GTCGAAT81450.08.7247511
CGGGAAT61400.08.4950511
GTCGGAT96500.08.4523351
CGGACAT36450.08.45148851
GCCGGAT50250.08.359751
GCCGAAT33450.08.2675921
CTCGGAT90600.08.0368011
CGGCAAT64500.08.0324881
CCCACAT58100.08.0135251
CCGACAT57000.07.98392771
GCCAGAT64250.07.95477341
GGGGGAT31900.07.90114261
CCTACTA36500.07.862946534
Produced by FastQC (version 0.11.5)