FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_19_NxW_C7-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_19_NxW_C7-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28611459
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC11600.011.16373238
CGGGGAT47600.011.0308351
GCCGAAT35900.010.92062951
CGGGCAT30550.010.8852021
CGGGAAT80900.010.7739491
GCGGGAT46600.010.3661441
GCCGGAT62600.09.95336151
CGCGCAC14100.09.68079840
GCGCGCA14250.09.333281539
CCGGCAT47150.09.2058531
TGCGCTA29550.08.883267
GCGGGGT16600.08.85656451
GTCGGAT113150.08.8478051
GCGGAAT59050.08.8326391
CGCCCAT48250.08.7783351
GCGGGCC8051.4133548E-98.6967741
CGCGGGT24150.08.5518271
CGGCAAT67000.08.5160251
GGGGGAT44500.08.4954791
CCCACAT71850.08.4283671
Produced by FastQC (version 0.11.5)