FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_18_NxW_C6-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_18_NxW_C6-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14778841
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT22000.012.0923741
CGGGCAT17850.011.96223451
CGCGTAT11000.011.7741531
GGGGGAT26550.011.0747881
CCGGCAT25600.010.1184131
CGGGAAT41100.010.0498791
CGCCCCT7859.094947E-129.8101051
GCCAGAT41650.09.4129051
CGGGGCT14550.09.382581
CGCCCAT24450.09.3058311
CCGGGAT38950.09.1667071
GCCGGAT29850.09.1468361
GTCGAAT58600.08.9003951
CGCGGAT20550.08.857521
GGGGATA35750.08.6162242
CGGCAAT34150.08.5076191
CCCCACT16600.08.4347571
CGCAGAT33000.08.2737281
CGGGCAC13550.08.2666851
CCCGTAT14500.08.20789051
Produced by FastQC (version 0.11.5)