FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_17_NxW_C6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_17_NxW_C6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48202981
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT54750.012.9153651
CGCGTAT37950.012.6371231
GGCGCGC27000.010.888803538
CGCGCAC28200.010.54956240
CGCCAAT99450.010.24301051
CGCCCAT84050.09.7458011
GGGGGAT75000.09.6149131
CGGGGAT75450.09.5575681
CGGGAAT129000.09.5519571
TAAACGT149050.09.13513853
CGCCCTT44500.08.9677661
GTGTGCG40200.08.9676098
CGGCAAT114500.08.8966581
CCGGCAT74550.08.6399171
CCGGGAT126100.08.6056971
ATCGGGC40100.08.5536116
TGCACTA149450.08.52452322
GCGCGCA35900.08.48182839
GTCGAAT213600.08.4236681
GCCAGAT138150.08.2858351
Produced by FastQC (version 0.11.5)