FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_16_NxW_C6-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_16_NxW_C6-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18233726
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT21000.010.5014821
CGGGGAT28400.09.8605471
CGCCCAT31800.09.3566671
GCCGGAT39950.09.287921
GCGGAAT35400.09.1962141
GCGGGAT36650.08.9780751
CGGGAAT46250.08.9309911
CCGGGAT43700.08.8113131
CCCACAT45250.08.5868471
CGCGTAT12300.08.537791
CGCCAAT39850.08.520651
GGGGGAT30350.08.4196411
GTCGGAT73350.08.3992781
CCCGCAT19850.08.2883241
CCCGTAT20350.08.2566941
GCCGAAT24600.08.2531981
GCGAAAT47800.08.1287631
CCCGCCT22000.08.1147821
GAAAAAT167850.07.9457151
ACCGCTA30950.07.91593949
Produced by FastQC (version 0.11.5)