FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_15_NxW_C5-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_15_NxW_C5-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11719304
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT13350.015.2086311
CGGGGAT20100.011.3203711
GCGGGGT6151.0004442E-1010.8148831
CGCCCAT19050.010.4742571
CGCGGAT15850.010.3803481
GGGGGAT16950.010.3262751
CGGGAAT32050.010.0485451
GGGGGAC5703.9481165E-89.8262651
GCCCGAT8202.1827873E-119.3918721
CGCGTAT8759.094947E-129.2015951
CCGGGAT29700.09.1935141
GCCGGAT25200.09.1682571
GCGGGAT20650.08.9846091
CCGGGGT27600.08.8783511
GCCGAAT15000.08.8682041
CGGGGAG15100.08.8094751
GGGGATA23200.08.6004462
TCGGGGG13450.08.5886662
CGGGGGT5351.719562E-58.5061481
GGGCGCT4200.00101773768.3347791
Produced by FastQC (version 0.11.5)