FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_14_NxW_C5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_14_NxW_C5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14619787
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT24650.011.92865851
CGGGCAT16850.011.2181981
CGCGGAT20650.010.6794711
CGGGAAT39400.010.128331
GCCGAAT17450.010.0300671
CGCGGGT13500.09.853241
CCCGCCT17800.09.8328471
CCGGCAT25650.09.8259461
CGCGCAC5006.939299E-79.7999440
CGGGGTT20150.09.7284181
CGGGGAG20250.09.6803761
CGGGGGT6201.4115358E-89.5981261
GCGGAAT28550.09.440911
GCCGGAT32400.09.0753531
GCGGGAT28100.08.8446631
CGGCAAT34150.08.7127931
GCCAGAT45400.08.6355781
GCGAAAT39250.08.5617161
CGCGTAT10303.6379788E-128.4963431
GGGGGGT5058.118551E-58.3180041
Produced by FastQC (version 0.11.5)