FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_13_NxW_C5-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_13_NxW_C5-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56610113
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT74100.011.5739841
CGGCAAT129950.09.8591581
CGGGCGC28450.09.84175415
GGGGGAT89650.09.6835891
CGGGGAT92550.09.60711
GCCGGAT126000.09.5848111
CCGGCAT92600.09.4128981
CGGGAAT153350.09.3819481
CCGGGAT145200.09.2576261
CGCGTAT41200.09.1761711
GCGGGAT133400.09.053121
CGCCCAT90900.08.934271
CGCGGAT90250.08.9210421
ATCGGGC53100.08.8323796
GCGGAAT112650.08.3901081
GCCAGAT175050.08.3788981
GTCGAAT203400.08.226441
CCGGGGT128500.07.98177531
GGGAGAT338550.07.92028761
GCGGGTT120100.07.89880231
Produced by FastQC (version 0.11.5)