FastQCFastQC Report
Tue 28 Jan 2020
HJMJ5DRXX_n01_12_NxW_C4-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJMJ5DRXX_n01_12_NxW_C4-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45377999
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT83150.011.7457911
CGGGCAT53950.011.3549941
CGGGAAT119150.010.6648041
GCGGGAT75000.010.4083991
GCGGAAT97000.09.5995351
CCGGCAT75850.09.2764271
CGGGGAG56600.09.0916191
CGCGGAT62450.09.0807741
CCGGGAT118200.08.9735741
CGGGGGT20700.08.9628441
GCCGAAT61250.08.7443121
GCGGGCG22050.08.571362566
CCCGCAT41250.08.5711231
GGGGGAT70000.08.5014141
GCGGGGT27850.08.4215091
GCCGGAT107350.08.315311
CGCCCAT64550.08.2972761
GGGAGAT252800.08.2391081
CGCGGGG19250.08.183181
GCCAGAT142050.08.1322931
Produced by FastQC (version 0.11.5)